In 2017, Illumina announced new sequencers, NovaSeq 6000, that could potentially reduces the cost of human genome sequencing to $100 in the future. NovaSeq is assembled with a new set of flowcells: S1, S2, S3, and S4. Both the systems can work with either one or two flow cells. In addition, NovaSeq 6000 can do 2×50 bp, 2×100 bp, and 2×150 bp read length using flow cells S1 and S2, pretty similar to the existing Illumina machines.
NovaSeq 6000系统通过突破性的创新开启了测序的新时代,为用户提供了比以往更快、更经济地完成项目的吞吐量、速度和灵活性,这些系统是从头开始构建的,为任何类型的测序提供了可扩展性和灵活性,无论是大规模测序还是靶向测序。
The NovaSeq Series lets you take your research further with a wide range of projects sizes for diverse applications—all on a single system. This platform redefines high throughput sequencing with unrivaled throughput, ease of use, low per sample costs, and unmatched flexibility. Whether you are interested in higher output applications for larger-scale projects or lower output applications for smaller-scale projects, the NovaSeq Series will empower you with new possibilities. Watch whole-genome and whole-exome sequencing demonstrations and learn how the NovaSeq Series can meet your lab’s evolving needs
亮点:
HiSeq X系统性能参数
| 流动池类型 | 岸上宪兵 | S1 | 第2页 | 第四心音 |
| 每个流动池的车道 | 2. | 2. | 2. | 2. |
| 每个流动池的输出 | ||||
| 2×50 bp | 65-80Gb | 134-167 Gb | 333-417 Gb | 不适用 |
| 2×100 bp | 不适用 | 266-333 Gb | 667-833 Gb | 1600-2000 Gb |
| 2×150 bp | 200-250 Gb | 400-500 Gb | 1000-1250Gb | 2400-3000 Gb |
| 单读 | 0.65-0.8 B | 1.3-1.6 B | 3.3-4.1 B | 8-10b |
| 配对末端读取 | 1.3-1.6 B | 2.6-3.2 B | 6.6-8.2 B | 16-20b |
| 质量分数 | ||||
| 2×50 bp | ≥ 85% | |||
| 2×100 bp | ≥ 80% | |||
| 2×150 bp | ≥ 75% | |||
| 运行时间 | ||||
| 2×50 bp | 约13小时 | 约13小时 | ~16小时 | 不适用 |
| 2×100 bp | 不适用 | 约19小时 | 约25小时 | 约36小时 |
| 2×150 bp | 约25小时 | 约25小时 | 约36小时 | ~44小时 |