靶向RNA测序

Targeted RNA-sequencing selects and sequences subset transcripts of interests, increasing the coverage of a focused set of RNA sequences. It is an accurate method that generates genomic and gene-expression information of specific genome regions. The specific RNA sequences analysis enables 测序 with high sensitivity and cost-effective access to NGS. By either targeted capture enrichment or amplicon-based approaches, Targeted RNA-Seq measures dozens to thousands of targets simultaneously. Enrichment assays also is a tool to identify both known and novel gene fusion partners in many sample types.

Providing qualitative and quantitative information for differential expression analysis, allele-specific expression measurement, and gene fusion verification, targeted RNA-sequencing can help to understand tumor classification and progression, genetic diseases and RNA drug response. Cancer transcriptome sequencing provides valuable information about gene expression changes in tumors. Profiling RNA-based drug response biomarkers aids too uncover multiple drug-susceptible tumorigenic pathways and improve the efficiency and success rate of the drug development process.

靶向RNA测序的优势

• Provides a comprehensive analysis of the specific transcripts
• 选择经过验证的途径、细胞或疾病特异性面板
• 与低质量、福尔马林固定、石蜡包埋（FFPE）兼容
• Fast turnaround time and Highest data quality
• RNA转录物的链信息
• 有效转录组和途径分析 

Targeted RNA Sequencing workflow

我们提供集成的靶向RNA-Seq工作流程，简化了从文库制备到数据分析和生物解释的整个过程。

样品要求

• 建议浓度应为20ug/uL或更高。
• A minimum of 2μg of the total is required.
• A260:A280值应在1.8和2.0之间
• RNA应储存在无核酸酶的水中或RNA稳定的水中。
• RNA must be validated using an assay such as a BioAnalyzer RNA chip. RIN value ≥ 7.0
• 组织、细胞、血液和血液制品都是可以接受的。

排序

• Illumina高通量测序仪
• Flexible service options: single end or paired-end sequencing, optional reads number according to research goals.

数据分析

• 原始数据质量控制
• Alignment and TPM/RPKM/FPKM-based quantitation
• Expression analysis
• SNP/Indels统计
• 备选拼接分析
• GO和KEGG注释