CD genomics can help you analyze genetic variation efficiently to meet diverse research needs. By utilizing Affymetrix and Illumina array platforms, we support high-throughput, multiplex processing to meet diverse research needs., delivering high-quality data at a low per-sample cost.
SNP微阵列简介
博凯森生物由最先进的变异检测和SNP基因分型技术提供支持，提供各种基因分型服务，以低的每样本成本提供高质量的数据。微阵列是一种有价值的工具，使研究人员能够探索从发现应用到常规筛查的单核苷酸多态性（SNP）和大结构变化等遗传变异。
Affymetrix and Illumina genotyping microarrays both provide trusted performance with respect to precision medicine initiatives, clinical and translational research, pharmacology, consumer screening, and agricultural applications. Based on the species and the selection of SNPs, multiple genotyping strategies are available.
表1。我们现有的自动化SNP基因分型平台。
图1。我们的HLA分型工作流程。
服务规范
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GeneTitan instrument together with Axiom array plates |
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GeneChip Scanner 3000 7G System together with Affymetrix GeneChip |
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Illumina iScan系统和Illumina BeadChips |
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SNP微阵列的优点
- 定制、灵活、可扩展
- 通话率>99%，准确率高
- 具有成本效益和高通量
- 在靶向或全基因组尺度上识别SNP
- 除了SNP，还可以测量其他遗传差异，如拷贝数变异
- 在生物标志物发现和验证、临床测试、GWAS、药物基因组学、法医学和品种鉴别中的应用
SNP Microarray Workflow
The general workflow for SNP Microarray is outlined below. We have three well-recognized genotyping platforms (Table 1) for genome-wide or targeted identification of SNPs and copy number variations in a high-throughput and affordable manner. Our highly experienced expert team executes quality management, following every procedure to ensure confident and unbiased results.
服务规范
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样品要求
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SNP分型
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生物信息学分析
我们提供定制的生物信息学分析，包括：
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我们的全方位服务涵盖了从基因组DNA的质量控制到基因型测定的整个过程。此外，博凯森生物还可以帮助您创建自己的定制微阵列，这些微阵列可以根据特定的项目要求进行定制。如果您有其他要求或问题，请随时与我们联系。
1.引入Illumina和Affymetrix基因分型平台。
表1。Affymetrix和Illumina基因分型解决方案。
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Affymetrix genotyping solutions |
The Affymetrix SNP microarray applies a combination of photolithography and combinatorial chemistry to directly synthesize oligonucleotides on a glass surface. Affymetrix arrays can achieve very high density to accommodate millions of probes on a single chip. |
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Illumina genotyping solutions |
Illumina’s unique BeadArray technology is based on 3-micron silica beads that self-assemble in micro-wells on planar silica slides. Each bead is covered with hundreds of thousands of copies of a specific oligonucleotide, which acts as the capture sequence. Accuracy and reliability of the assays are usually above 99.5%. |
2.如何根据我的特定需求选择最合适的基因分型平台？
The choice of genotyping strategies depends on the species, the purpose, the number of SNPs per sample, and the number of samples. For different cases, we recommend different genotyping technologies.
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- 给 全基因组SNP基因分型，我们推荐Illumina BeadChips、Affymetrix GeneChips或 天然气.
- 对于全基因SNP基因分型，如候选区域的精细定位或单倍型分型，我们建议使用NGS或基于阵列的技术。
- 对于单个SNP的分析，我们建议使用实时PCR检测、Sanger测序、Illumina BeadChips、Affymetrix技术或 天然气.
3.博凯森生物提供哪些阵列？
在博凯森生物，我们为您的SNP基因分型项目提供现成和定制的阵列。
表2。我们现有的SNP基因分型阵列。
| 人 | 动物 | 植物 |
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